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To measure the zone of inhibition, first place the plate on a non-reflective surface. Take a ruler or caliper that measures in millimeters and place the “0” in the center of the antibiotic disk. Measure from the center of the disk to the edge of area with zero growth. Take your measurement in millimeters.Click to see full answer. Subsequently, one may also ask, what does zone of inhibition indicate?A larger zone of inhibition around an antibiotic-containing disk indicates that the bacteria are more sensitive to the antibiotic in the disk. If the observed zone of inhibition is greater than or equal to the size of the standard zone, the microorganism is considered to be sensitive to the antibiotic.Additionally, what does zone of inhibition mean in microbiology? Definition. (microbiology) The clear region around the paper disc saturated with an antimicrobial agent on the agar surface. Supplement. The clear region is an indication of the absence, or the effective inhibition, of microbial growth by the antimicrobial agent. ( Keeping this in view, how is Kirby Bauer’s zone of inhibition measured? Place the metric ruler across the zone of inhibition, at the widest diameter, and measure from one edge of the zone to the other edge. HOLDING THE PLATE UP TO THE LIGHT MIGHT HELP. Use millimeter measurements. The disc diameter will actually be part of that number.Why is it called the zone of inhibition?If an antibiotic stops the bacteria from growing or kills the bacteria, there will be an area around the wafer where the bacteria have not grown enough to be visible. This is called a zone of inhibition.

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